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Another recurring limitation emerges on antioxidant-based interventions in ASD, constrained by a fundamental methodological gap: most clinical studies report behavioral or functional improvements without directly measuring oxidative-stress biomarkers

For the azido-tagged small molecules screen, 90 L of the cell culture was added into wells containing 10 L of the azido-tagged test compound at 500 M in 96-well plates, covered with parafilm, and incubated for 1 h at 37 C in a shaker incubator

It is important to note that taking expired medication once is generally not considered a medical emergency, but it should not become routine practice

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